Culture-Independent Phylogenetic Analysis of the Microbial Community in Industrial Sugarcane Bagasse Feedstock Piles
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- RATTANACHOMSRI Ukrit
- Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology
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- KANOKRATANA Pattanop
- Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology
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- EURWILAICHITR Lily
- Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology
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- IGARASHI Yasuo
- Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- CHAMPREDA Verawat
- Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology
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Abstract
Sugarcane bagasse is an important lignocellulosic by-product with potential for conversion to biofuels and chemicals in biorefinery. As a step towards an understanding of microbial diversity and the processes existing in bagasse collection sites, the microbial community in industrial bagasse feedstock piles was investigated. Molecular biodiversity analysis of 16S rDNA sequences revealed the presence of a complex bacterial community. A diverse group of mainly aerobic and facultative anaerobic bacteria was identified reflecting the aerobic and high temperature microenvironmental conditions under the pile surface. The major bacterial taxa present were identified as Firmicutes, Alpha- and Gammaproteobacteria, Acidobacteria, Bacteroidetes, and Actinobacteria. Analysis of the eukaryotic microbial assemblage based on an internal transcribed spacer revealed the predominance of diverse cellulolytic and hemicellulolytic ascomycota. A microbial interaction model is proposed, focusing on lignocellulose degradation and methane metabolism. The insights into the microbial community in this study provide a basis for efficient utilization of bagasse in lignocellulosic biomass-based industries.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (2), 232-239, 2011
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681455162752
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- NII Article ID
- 10027897471
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 10993145
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed