Cardioprotective Effects of 3-Phosphoinositide-Dependent Protein Kinase-1 on Hypoxic Injury in Cultured Neonatal Rat Cardiomyocytes and Myocardium in a Rat Myocardial Infarct Model
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- SHIM Tae-Jin
- Department of Internal Medicine, College of Medicine, Chungbuk National University College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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- BAE Jang-Whan
- Department of Internal Medicine, College of Medicine, Chungbuk National University
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- KIM Yeon-Jung
- Department of Internal Medicine, College of Medicine, Chungbuk National University
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- KIM Dae Joong
- College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University
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- HWANG Kyung-Kuk
- Department of Internal Medicine, College of Medicine, Chungbuk National University
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- KIM Dong-Woon
- Department of Internal Medicine, College of Medicine, Chungbuk National University
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- CHO Myeong-Chan
- Department of Internal Medicine, College of Medicine, Chungbuk National University
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Abstract
3-Phosphoinositide-dependent protein kinase-1 (PDK1) is involved in numerous cellular responses. In this study, we investigated the protective effects of PDK1 gene expression against hypoxic conditions in cultured rat CMCs (rCMCs) and in a rat myocardial infarction (MI) model using the lentiviral vector (LeV) system. LeV-PDK1 transfer effectively reduced the apoptotic cell death caused by hypoxic injury as compared to LeV-GFP transfer in rCMCs the expression of survival proteins increased in the LeV-PDK1 group, whereas apoptosis signaling decreased in the rCMCs and in infarcted hearts treated with LeV-PDK1. LeV-PDK1 transfer also reduced apoptosis and infarct size and attenuated myocardial wall thinning and ventricular remodeling in a rat MI model. These findings suggest that PDK1 has a protective role in the injured ischemic myocardium via overexpression of the cell survival pathway in CMCs. Hence PDK1 can be used as a treatment strategy for myocardial salvage inin hypoxic injury.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (1), 101-107, 2012
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681455217664
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- NII Article ID
- 10030398626
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 023407631
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed