Characterization of Coumarin-Specific Prenyltransferase Activities in <i>Citrus limon</i> Peel

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  • MUNAKATA Ryosuke
    Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University
  • INOUE Tsuyoshi
    Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University
  • KOEDUKA Takao
    Institute for Chemical Research, Kyoto University Institute for Chemical Research, Kyoto University
  • SASAKI Kanako
    Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University
  • TSURUMARU Yusuke
    Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University
  • SUGIYAMA Akifumi
    Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University
  • UTO Yoshihiro
    Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima
  • HORI Hitoshi
    Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima Department of Life System, Institute of Technology and Science, Graduate School, The University of Tokushima
  • AZUMA Jun-ichi
    Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University
  • YAZAKI Kazufumi
    Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University Laboratory of Plant Gene Expression, Research Institute for Sustainable Humanosphere, Kyoto University

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  • Characterization of Coumarin-Specific Prenyltransferase Activities in Citrus limon Peel

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Coumarins, a large group of polyphenols, play important roles in the defense mechanisms of plants, and they also exhibit various biological activities beneficial to human health, often enhanced by prenylation. Despite the high abundance of prenylated coumarins in citrus fruits, there has been no report on coumarin-specific prenyltransferase activity in citrus. In this study, we detected both O- and C-prenyltransferase activities of coumarin substrates in a microsome fraction prepared from lemon (Citrus limon) peel, where large amounts of prenylated coumarins accumulate. Bergaptol was the most preferred substrate out of various coumarin derivatives tested, and geranyl diphosphate (GPP) was accepted exclusively as prenyl donor substrate. Further enzymatic characterization of bergaptol 5-O-geranyltransferase activity revealed its unique properties: apparent Km values for GPP (9 µM) and bergaptol (140 µM) and a broad divalent cation requirement. These findings provide information towards the discovery of a yet unidentified coumarin-specific prenyltransferase gene.

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