Differential Expression of Virulence and Stress Fitness Genes during Interaction between <I>Listeria monocytogenes</I> and <I>Bifidobacterium longum</I>
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- TAN Qianglai
- State Key Laboratory of Food Science and Technology, Nanchang University State Key Laboratory of Food Science and Technology, Nanchang University
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- XU Hengyi
- State Key Laboratory of Food Science and Technology, Nanchang University State Key Laboratory of Food Science and Technology, Nanchang University
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- CHEN Tingtao
- Jiangxi-OAI Joint Research Institute, Nanchang University Jiangxi-OAI Joint Research Institute, Nanchang University
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- LI Peng
- State Key Laboratory of Food Science and Technology, Nanchang University State Key Laboratory of Food Science and Technology, Nanchang University
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- AGUILAR Zoraida P.
- Ocean NanoTech LLC Ocean NanoTech LLC
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- XU Di
- State Key Laboratory of Food Science and Technology, Nanchang University State Key Laboratory of Food Science and Technology, Nanchang University
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- MING Xing
- State Key Laboratory of Food Science and Technology, Nanchang University State Key Laboratory of Food Science and Technology, Nanchang University
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- XU Feng
- Jiangxi-OAI Joint Research Institute, Nanchang University Jiangxi-OAI Joint Research Institute, Nanchang University
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- WEI Hua
- State Key Laboratory of Food Science and Technology, Nanchang University State Key Laboratory of Food Science and Technology, Nanchang University
Bibliographic Information
- Other Title
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- Differential Expression of Virulence and Stress Fitness Genes during Interaction between Listeria monocytogenes and Bifidobacterium longum
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Abstract
Bifidobacterium is well known to have an inhibitory effect on the survival, growth, and proliferation of various foodborne pathogens, but the mechanism of the molecular action of B. longum in blocking the invasion of Listeria monocytogenes is not yet well defined. In the present study, following RNA extraction and cDNA synthesis, differential expression of virulence and stress fitness genes in L. monocytogenes and B. longum was determined by real-time PCR. The results indicate that L. monocytogenes virulence factors, including actA, hly, inlA, and plcA, showed significantly downregulated expression during co-incubation of B. longum and L. monocytogenes in phosphate-buffered saline. The relative mRNA levels of oppA and serpin, two stress fitness genes in B. longum, were significantly higher than for the control group. These results indicate that downregulation of L. monocytogenes virulence factors during co-incubation with B. longum might be responsible for the inhibitory effects.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (4), 699-704, 2012
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681455290112
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- NII Article ID
- 10030751538
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC38rjt1WjtA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 023592906
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- PubMed
- 22484935
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed