Construction of a Metagenomic Library for the Marine Sponge Halichondria okadai
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- ABE Takahiro
- Department of Chemistry, Faculty of Science, Kanagawa University Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University Department of Chemistry, Faculty of Science, Kanagawa University Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
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- SAHIN Fatma Pinar
- Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University
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- AKIYAMA Kiyotaka
- Molecular Neuroscience Unit, Okinawa Institute of Science and Technology Molecular Neuroscience Unit, Okinawa Institute of Science and Technology
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- NAITO Takayuki
- Molecular Neuroscience Unit, Okinawa Institute of Science and Technology Molecular Neuroscience Unit, Okinawa Institute of Science and Technology
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- KISHIGAMI Mizoe
- Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
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- MIYAMOTO Kenji
- Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
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- SAKAKIBARA Yasufumi
- Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
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- UEMURA Daisuke
- Department of Chemistry, Faculty of Science, Kanagawa University Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University Department of Chemistry, Faculty of Science, Kanagawa University Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
書誌事項
- タイトル別名
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- Construction of a Metagenomic Library for the Marine Sponge <I>Halichondria okadai</I>
- Construction of a metagenomic library for the marine dponge Halichondria okadai
この論文をさがす
抄録
Symbionts of the marine sponge Halichondria okadai are promising as a source of natural products. Metagenomic technology is a powerful tool for accessing the genetic and biochemical potential of bacteria. Hence, we established a method of recovering bacterial-enriched metagenomic DNA by stepwise centrifugation. The metagenomic DNA was analyzed by ultrafast 454-pyrosequencing technology, and the results suggested that more than three types of bacterial DNA, Alphaproteobacteria, Actinobacteria, and Cyanobacteria, had been recovered, and that eukaryotic genes comprised only 0.02% of the metagenomic DNA. These results indicate that stepwise centrifugation and real-time quantitative PCR were effective for separating sponge cells and symbiotic bacteria, and that we constructed a bacteria-enriched metagenomic library from a marine sponge, H. okadai, selectively for the first time.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (4), 633-639, 2012
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681455312768
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- NII論文ID
- 10030751181
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC38rjt1Sqtg%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 023592723
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- PubMed
- 22484923
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可