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Glycosaminoglycan and Collagen Facilitate the Degradation of Pigment Epithelium-Derived Factor by Chymotrypsin
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- SAITO Akio
- Division of Basic Medicine, Faculty of Medicine, Kinki University
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Description
Pigment epithelium-derived factor (PEDF) is a member of the serine protease inhibitor family. It is present in a variety of tissues and organs, including plasma. Here, PEDF was purified from human plasma by use of a dermatan sulfate affinity column, and then hydroxyapatite, gel filtration and ion exchange columns. It did not form a complex with various proteases, including chymotrypsin, elastase, kallikrein, thrombin, plasmin, cathepsins G, activated protein C, and urokinase, but collagen type I facilitated the degradation of PEDF by chymotrypsin more than 10-fold. Dermatan sulfate, heparan sulfate, and heparin showed similar effects, but other glycosaminoglycans, such as hyaluronic acid, chondroitin sulfate A, C, D, E, and keratan sulfate, had no effect on PEDF degradation.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (8), 1628-1632, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681455407488
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- NII Article ID
- 10031195808
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sfns1OmtA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 024791587
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- PubMed
- 23924722
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed