Formulation of a Universal First-Order Rate Constant for Enzymatic Reactions
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- IMOTO Taiji
- Emeritus Professor, Graduate School of Pharmaceutical Sciences, Kyushu University
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Abstract
It is a common practice to employ kcat[E]0/Km as a first-order rate constant for the analysis of an enzymatic reaction, where [E]0 is the total enzyme concentration. I describe in this report a serious shortcoming in analyzing enzymatic reactions when kcat[E]0/Km is employed and show that kcat[E]0/Km can only be applied under very limited conditions. I consequently propose the use of a more universal first-order rate constant, kcat[ES]K/[S]0, where [ES]K is the initial equilibrium concentration of the ES-complex derived from [E]0, [S]0 and Km. Employing kcat[ES]K/[S]0 as the first-order rate constant enables all enzymatic reactions to be reasonably simulated under a wide range of conditions, and the catalytic and binding contributions to the rate constant of any enzyme can be determined under any and all conditions.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (8), 1703-1708, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681455470848
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- NII Article ID
- 10031195820
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sfns1OltQ%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 024791817
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- PubMed
- 23924733
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed