Improvement of L-Lactate Production by CYB2 Gene Disruption in a Recombinant Saccharomyces cerevisiae Strain under Low pH Condition

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  • OOKUBO Aki
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University
  • HIRASAWA Takashi
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University
  • YOSHIKAWA Katsunori
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University
  • NAGAHISA Keisuke
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University
  • FURUSAWA Chikara
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University
  • SHIMIZU Hiroshi
    Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University

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  • Improvement of<scp>L</scp>-Lactate Production by<i>CYB2</i>Gene Disruption in a Recombinant<i>Saccharomyces cerevisiae</i>Strain under Low pH Condition

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Using a DNA microarray, we found that expression of the genes related to lactate metabolism was upregulated in a lactate-producing recombinant Saccharomyces cerevisiae strain. Disruption of the CYB2 gene encoding L-lactate dehydrogenase improved the L-lactate production by S. cerevisiae under low pH condition.

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