Differences in the Roles of a Glutamine Amidotransferase Subunit of Pyridoxal 5'-Phosphate Synthase between <i>Bacillus circulans</i> and <i>Bacillus subtilis</i>
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- ITAGAKI Shiori
- Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
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- HAGA Minami
- Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
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- OIKAWA Yuji
- Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
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- SAKODA Ayaka
- Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
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- OHKE Yoshie
- Department of Chemistry, College of Humanities and Sciences, Nihon University
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- SAWADA Hiroshi
- Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University Department of General Studies, College of Humanities and Sciences, Nihon University
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- EGUCHI Tadashi
- Department of Chemistry and Materials Science, Graduate School of Science and Engineering, Tokyo Institute of Technology
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- TAMEGAI Hideyuki
- Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University Department of Chemistry, College of Humanities and Sciences, Nihon University
Bibliographic Information
- Other Title
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- Differences in the Roles of a Glutamine Amidotransferase Subunit of Pyridoxal 5'-Phosphate Synthase between Bacillus circulans and Bacillus subtilis
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Abstract
BtrC2 of the butirosin producer Bacillus circulans is a non-catalytic subunit of 2-deoxy-scyllo-inosose (DOI) synthase that is involved in butirosin biosynthesis, and also a homolog of glutamine amidotransferase subunit (PdxT) of pyridoxal 5'-phosphate (PLP) synthase of Bacillus subtilis. BtrC2 has been found to have functions in B. circulans both in primary and secondary metabolism. In this study, we investigated the properties of PdxT of B. subtilis in order to determine whether the property of enzyme stabilization is universal among PdxT homologs. Complementation with PdxT in the btrC2 disruptant of B. circulans restored the growth and short-term production of antibiotics, but long-term production of antibiotics cannot be restored. Additionally, PdxT did not bind physically with or stabilize BtrC. Our results indicate that the function of BtrC2 in secondary metabolism is specific properties, not universal among PdxT homologs.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (7), 1481-1485, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681455594112
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- NII Article ID
- 10031190456
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sjos1ehtQ%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 024723608
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- PubMed
- 23832367
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed