High-Yield Expression of Mouse Aos1-Uba2-Fusion SUMO-Activating Enzyme, mAU, in a Baculovirus-Insect Cell System
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- KANEMARU Ayumi
- Faculty of Sciences, Kumamoto University
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- SAITOH Hisato
- Faculty of Sciences, Kumamoto University Department of New Frontier Sciences, Graduate School of Science and Technology, Kumamoto University
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Abstract
Small ubiquitin-related modifier (SUMO) research requires large amounts of SUMO-activating enzyme (SUMO-E1) for in vitro SUMOylation assays. In this study the expression and purification of a hexahistidine-tagged mouse Aos1-Uba2-fusion protein (His6-mAU) in a baculovirus-insect cell system revealed that approximately 1.0 mg of highly-purified His6-mAU was obtained from 100 mL of Sf9 cell culture. The recombinant protein had SUMO-E1 activity in multiple in vitro SUMOylation assays.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (7), 1575-1578, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681455619840
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- NII Article ID
- 10031190472
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sjos1aqsQ%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 024723801
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- PubMed
- 23832333
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed