Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster

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  • JUNG Hye-Jin
    Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
  • PARK Sung-Hee
    Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
  • HA Sang-Do
    Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
  • LEE Kyu-Ho
    Department of Environmental Engineering and Biotechnology, Hankuk University of Foreign Studies
  • CHUNG Duck Hwa
    Division of Applied Life Science, Gyeongsang National University
  • KIM Cheol-Ho
    Department of Biological Sciences, College of Natural Science, Sungkyunkwan University
  • KIM Min-Gon
    Laboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology
  • KIM Kwang-Yup
    Department of Food Science and Technology, Chungbuk National University
  • KIM Keun-Sung
    Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University

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  • Species-Specific Detection of<i>Listeria monocytogenes</i>Using Polymerase Chain Reaction Assays Targeting the<i>prfA</i>Virulence Gene Cluster

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Description

Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.

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