X-ray crystal structure of the DNA-binding domain of response regulator WalR essential to the cell viability of Staphyloccus aureus and interaction with target DNA
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- DOI Akihiro
- Department of Bioscience, Graduate School of Agriculture, Kinki University
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- OKAJIMA Toshihide
- Institute of Scientific and Industrial Research, Osaka University
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- GOTOH Yasuhiro
- Department of Bioscience, Graduate School of Agriculture, Kinki University
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- TANIZAWA Katsuyuki
- Institute of Scientific and Industrial Research, Osaka University
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- UTSUMI Ryutaro
- Department of Bioscience, Graduate School of Agriculture, Kinki University
書誌事項
- タイトル別名
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- X-Ray Crystal Structure of the DNA-Binding Domain of Response Regulator WalR Essential to the Cell Viability of Staphylococcus aureus and Interaction with Target DNA
- X-Ray Crystal Structure of the DNA-Binding Domain of Response Regulator WalR Essential to the Cell Viability of <i>Staphylococcus aureus</i> and Interaction with Target DNA
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A bacterial two-component signal transduction system, WalK/WalR, is essential to the cell viability of Gram-positive bacteria and is therefore a potential target for the development of a new class of antibiotics. We have solved the X-ray crystal structure of the DNA-binding domain of the response regulator WalR (WalRc) from a Gram-positive pathogen Staphylococcus aureus, currently causing serious problems in public health through the acquisition of multi-drug resistance. The structure contains a winged helix-turn-helix motif and closely resembles those of WalRs of Bacillus subtilis and Enterococcus faecalis, and also that of PhoB of Escherichia coli. Gel mobility shift assays with mutant WalRs revealed specific interactions of WalR with the target DNA, as elaborated by in silico modeling of the WalRc-DNA complex.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (9), 1901-1907, 2010
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681455641856
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- NII論文ID
- 10027559722
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10833940
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可