Molecular Cloning and Sequence Analysis of Two Distinct Halotolerant Extracellular Proteases from Bacillus subtilis FP-133

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  • TAKENAKA Shinji
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • YOSHIDA Nobutaka
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • YOSHIDA Ken-ichi
    Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • MURAKAMI Shuichiro
    Department of Bioscience, Biotechnology, and Agrochemistry, Faculty of Agriculture, Meiji University
  • AOKI Kenji
    Department of Nutritional Management, Faculty of Nutritional Science, Sagami Women’s University

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  • Molecular Cloning and Sequence Analysis of Two Distinct Halotolerant Extracellular Proteases from<i>Bacillus subtilis</i>FP-133

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Abstract

We cloned the genes encoding the two distinct extracellular halotolerant proteases of Bacillus subtilis FP-133 Expro-I and Expro-II, which were classified as alkaline serine and neutral proteases respectively. Three-dimensional modeling suggested that acidic and polar amino acid residues located on the surface stabilize protein structure in the presence of relatively high NaCl concentrations.

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