Improvement of the Transformation Efficiency of<i>Flammulina velutipes</i>Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter

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  • MAEHARA Tomoko
    Food Biotechnology Division, National Food Research Institute
  • TOMITA Shizuko
    Food Biotechnology Division, National Food Research Institute
  • TAKABATAKE Koji
    Forest Institute, Toyama Prefectural Agricultural, Forestry, and Fisheries Research Center
  • KANEKO Satoshi
    Food Biotechnology Division, National Food Research Institute

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  • Improvement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter

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To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control the expression of target genes. When the hygromycin B phosphotransferase (hph) gene from Escherichia coli was controlled by the gpd promoter, transformation efficiency was 3-fold higher than the case of that controlled by the tryptophan synthetase gene (trp1) promoter.

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