Practical Preparation of Lacto-N-biose I, a Candidate for the Bifidus Factor in Human Milk

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  • Practical preparation of lacto-N-biose 1, a candidate for the bifidus factor in human milk
  • Practical Preparation of Lacto-<i>N</i>-biose I, a Candidate for the Bifidus Factor in Human Milk
  • Practical preparation of lacto-N-biose I, the candidate of the bifidus factor in human milk
  • Practical preparation of lacto-<italic>N</italic>-biose I, a candidate for the bifidus factor in human milk

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Abstract

A one-pot enzymatic reaction to produce lacto-N-biose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500 mM of LNB was generated in 10-liter of reaction mixture initially containing 660 mM of sucrose and 600 mM of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDP-glucose—hexose-1-phospate uridylyltransferase, UDP-glucose 4-epimerase, and lacto-N-biose phosphorylase, in the presence of UDP-Glc and phosphate, indicating a reaction yield of 83%. LNB was isolated from the mixture by crystallization after yeast treatment. Finally, 1.4 kg of LNB of 99.6% purity was recovered after recrystallization.

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