Production and Characterization of a Biosurfactant from <i>Cyberlindnera samutprakarnensis</i> JP52<sup>T</sup>

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  • POOMTIEN Jamroonsri
    Department of Microbiology, Faculty of Science, Chulalongkorn University
  • THANIYAVARN Jiraporn
    Department of Microbiology, Faculty of Science, Chulalongkorn University
  • PINPHANICHAKARN Pairoh
    Department of Microbiology, Faculty of Science, Chulalongkorn University
  • JINDAMORAKOT Sasitorn
    Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC)
  • MORIKAWA Masaaki
    Environmental Molecular Biology Laboratory, Environmental Biology Section, Faculty of Environmental Earth Science, Hokkaido University

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  • Production and Characterization of a Biosurfactant from Cyberlindnera samutprakarnensis JP52[T]

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Abstract

Cyberlindnera samutprakarnensis JP52T, isolated from cosmetic industrial wastes in Thailand, was found to be an efficient biosurfactant-producing yeast when cultured in a medium containing (2% (w/v) glucose and 2% (v/v) palm oil at 30 °C, 200 rpm for 7 d. The crude biosurfactant had the ability to reduce the surface tension from 55.7 to 30.9 mN/m at 25 °C with a critical micelle concentration (CMC) of 0.046%. Physicochemical analysis of the crude biosurfactant revealed that it had wide ranges of optimum pH and pH stability at 6–9 and 3–10 respectively. It was also thermostable and retained 80% activity even after heat treatment, and it tolerated NaCl at 1.0–10%. Furthermore, it effectively emulsified various vegetable oils with an E24 value of over 80%. A partially purified biosurfactant fraction was analyzed for its structure by MALDI-TOF MS and NMR. This revealed that the biosurfactant mainly contained sophorolipids in C18-(MW 574) and C16-diaceltylated (MW 662) forms.

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