Production and Characterization of a Biosurfactant from <i>Cyberlindnera samutprakarnensis</i> JP52<sup>T</sup>
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- POOMTIEN Jamroonsri
- Department of Microbiology, Faculty of Science, Chulalongkorn University
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- THANIYAVARN Jiraporn
- Department of Microbiology, Faculty of Science, Chulalongkorn University
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- PINPHANICHAKARN Pairoh
- Department of Microbiology, Faculty of Science, Chulalongkorn University
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- JINDAMORAKOT Sasitorn
- Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC)
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- MORIKAWA Masaaki
- Environmental Molecular Biology Laboratory, Environmental Biology Section, Faculty of Environmental Earth Science, Hokkaido University
Bibliographic Information
- Other Title
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- Production and Characterization of a Biosurfactant from Cyberlindnera samutprakarnensis JP52[T]
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Abstract
Cyberlindnera samutprakarnensis JP52T, isolated from cosmetic industrial wastes in Thailand, was found to be an efficient biosurfactant-producing yeast when cultured in a medium containing (2% (w/v) glucose and 2% (v/v) palm oil at 30 °C, 200 rpm for 7 d. The crude biosurfactant had the ability to reduce the surface tension from 55.7 to 30.9 mN/m at 25 °C with a critical micelle concentration (CMC) of 0.046%. Physicochemical analysis of the crude biosurfactant revealed that it had wide ranges of optimum pH and pH stability at 6–9 and 3–10 respectively. It was also thermostable and retained 80% activity even after heat treatment, and it tolerated NaCl at 1.0–10%. Furthermore, it effectively emulsified various vegetable oils with an E24 value of over 80%. A partially purified biosurfactant fraction was analyzed for its structure by MALDI-TOF MS and NMR. This revealed that the biosurfactant mainly contained sophorolipids in C18-(MW 574) and C16-diaceltylated (MW 662) forms.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (12), 2362-2370, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681455829376
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- NII Article ID
- 130003381947
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC2c3ks1amsw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 025124152
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- PubMed
- 24317046
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed