Enzymatic Hydrolysis of Steam-Exploded Hardwood Using Short Processing Times
-
- HORN Svein J.
- Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences
-
- EIJSINK Vincent G. H.
- Department of Chemistry, Biotechnology, and Food Science, Norwegian University of Life Sciences
この論文をさがす
抄録
Woody materials are generally resistant to enzymatic saccharification unless they undergo harsh fiber disrupting pre-treatments such as steam explosion. In this study, enzymatic hydrolysis of steam-exploded hardwood was investigated using various conditions and commercial cellulase preparations. Hydrolysis times were kept below 24 h in order to approach industrially realistic conditions. The optimal conditions for enzymatic hydrolysis were found to be 55 °C and a pH of about 4.5. During enzymatic hydrolysis, acetate was released from the hemicellulose fraction. Increasing the dry matter concentration had a negative effect on glucose yields. Using fermentors with pH control, 66% of the cellulose in steam-exploded hardwood could be hydrolyzed to glucose within 12 h using an enzyme dose of 25 filter paper units (FPU)/g of dry matter. For 24 h hydrolysis maximum cellulose saccharification (71%) was observed at 25 FPU/g, and increasing the enzyme dose further did not increase the sugar yield.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 74 (6), 1157-1163, 2010
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681455830912
-
- NII論文ID
- 10027555946
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 10733922
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可