The Bovine Lactophorin C-Terminal Fragment and PAS6/7 Were Both Potent in the Inhibition of Human Rotavirus Replication in Cultured Epithelial Cells and the Prevention of Experimental Gastroenteritis

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  • INAGAKI Mizuho
    United Graduate School of Agricultural Science, Gifu University
  • NAGAI Sayaka
    Department of Applied Life Science, Gifu University
  • YABE Tomio
    Department of Applied Life Science, Gifu University
  • NAGAOKA Satoshi
    Department of Applied Life Science, Gifu University
  • MINAMOTO Nobuyuki
    Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • TAKAHASHI Takeshi
    Food Science Institute, Division of Research and Development, Meiji Dairies Co., Ltd.
  • MATSUDA Tsukasa
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
  • NAKAGOMI Osamu
    Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences and Global Center of Excellence, Nagasaki University
  • NAKAGOMI Toyoko
    Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences and Global Center of Excellence, Nagasaki University
  • EBINA Takusaburo
    Sendai Institute of Microbiology
  • KANAMARU Yoshihiro
    Department of Applied Life Science, Gifu University

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Rotaviruses are the leading cause of severe dehydrating diarrhea in children worldwide. We have found that high-Mr glycoprotein fraction (F1) from cow’s milk whey has potent inhibitory activity against human rotavirus (HRV) in cell culture. The present study was undertaken to identify and characterize the components responsible for this inhibitory activity. F1 was initially heated at 95 °C for 30 min, rendering milk antibodies inert, subjected to ammonium sulfate fractionation, and then resolved by two-dimensional polyacrylamide gel electrophoresis. After electroelution, we found that a heat-stable milk protein lactophorin C-terminal fragment (LP16) and bovine milk fat globule membrane protein PAS6/7 strongly inhibited the replication of HRV MO strains in MA104 cells. Furthermore, we found that prophylactic oral administration of F1 once before inoculation of the HRV MO strain obviously prevented the development of diarrhea in vivo. These non-immunoglobulin components are a promising candidate for a prophylactic food additive against HRV infection.

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