Structure and Expression Analysis of the<i>Cecropin-E</i>Gene from the Silkworm,<i>Bombyx mori</i>

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  • HONG Sun-Mee
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • KUSAKABE Takahiro
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • LEE Jae-Man
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • TATSUKE Tsuneyuki
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • KAWAGUCHI Yutaka
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • KANG Min-Wook
    Department of Agricultural Biology, NIAST, RDA
  • KANG Seok-Woo
    Department of Agricultural Biology, NIAST, RDA
  • KIM Kyung-A
    College of Agriculture and Life Sciences, Kyungpook National University
  • NHO Si-Kab
    College of Agriculture and Life Sciences, Kyungpook National University

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  • Structure and Expression Analysis of the Cecropin-E Gene from the Silkworm, Bombyx mori

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説明

Cecropins belong to the antibacterial peptides family and are induced after injection of bacteria or their cell-wall components. By silkworm cDNA microarray analysis, a novel type of Cecropin family gene was identified as a cDNA up-regulated in early embryo, 1 day after oviposition. The cDNA isolated was 394 bp with 198 ORF translating 65 amino acids, encoding BmCecropin-E (BmCec-E). Using Southern hybridization and genome search analysis, the number of BmCec-E gene was estimated to be at least two per haploid, which consisted of two exons, as in other Cecropin family members. BmCec-E mRNA was expressed transiently 1 day after egg-laying (AEL, germ-band formation stage), and was specifically expressed in the degenerating intestine during the pre-pupal and pupal stages, unlike other Cecropin family genes. Immune challenge analysis showed that BmCec-E gene expression was more strongly induced by Escherichia coli (gram-negative) than by Micrococus luteus (gram-positive), and not by virus injection. By bacterial challenge, expression of BmCec-E mRNA was induced 12 h after injection, and was maintained for 24 h. Expression of BmCec-E after immune challenge was observed strongly in excretory organs, such as hindgut and malphigian, slightly in fat body, skin, and midgut.

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