Comparative Study of the in Vitro Protective Effects of Several Antioxidants on Elongation Factor 2 under Oxidative Stress Conditions
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- ARGUELLES Sandro
- Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Seville
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- CANO Mercedes
- Department of Physiology and Zoology, Faculty of Pharmacy, University of Seville
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- MACHADO Alberto
- Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Seville
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- AYALA Antonio
- Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Seville
Bibliographic Information
- Other Title
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- Comparative Study of the<i>in Vitro</i>Protective Effects of Several Antioxidants on Elongation Factor 2 under Oxidative Stress Conditions
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Abstract
One of the biochemical pathways affected by aging in all organisms is protein synthesis. Previous reports from our laboratory have indicated that the elongation step is specially affected by aging as a consequence of alterations in elongation factor-2 (eEF-2). In the present work, we studied in vitro the effectiveness of several individual nutritional antioxidants in protecting the levels of hepatic eEF-2 subjected to oxidative stress induced by cumene hydroperoxide. The in vitro system employed consisted of rat liver homogenates treated with cumene hydroperoxide. The antioxidants used in this study were lipoic acid, coenzyme Q10, tethrahydrofolic acid, and N-tert-butyl-alpha-phenylnitrone. The results indicate that the antioxidants have different capacities to prevent eEF-2 loss, folic acid being the most effective. A comparison between the antioxidants used and their potential pro-oxidant activity is also discussed, on the basis of the oxidative stress parameters measured.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (7), 1373-1379, 2010
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681456090112
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- NII Article ID
- 10027557055
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 10766470
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed