Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol

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  • HABE Hiroshi
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
  • SHIMADA Yuko
    Department of Chemical Industry, Science University of Tokyo
  • FUKUOKA Tokuma
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
  • KITAMOTO Dai
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
  • ITAGAKI Masayuki
    Department of Chemical Industry, Science University of Tokyo
  • WATANABE Kunihiro
    Department of Chemical Industry, Science University of Tokyo
  • YANAGISHITA Hiroshi
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
  • YAKUSHI Toshiharu
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
  • MATSUSHITA Kazunobu
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
  • SAKAKI Keiji
    Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)

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  • Use of a<i>Gluconobacter frateurii</i>Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol

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To prevent dihydroxyacetone (DHA) by-production during glyceric acid (GA) production from glycerol using Gluconobacter frateurii, we used a G. frateurii THD32 mutant, ΔsldA, in which the glycerol dehydrogenase subunit-encoding gene (sldA) was disrupted, but ΔsldA grew much more slowly than the wild type, growth starting after a lag of 3 d under the same culture conditions. The addition of 1% w/v D-sorbitol to the medium improved both the growth and the GA productivity of the mutant, and ΔsldA produced 89.1 g/l GA during 4 d of incubation without DHA accumulation.

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