Molecular characterization of HbCDPK1, an ethephon-induced calcium-dependent protein kinase gene of Hevea brasiliensis

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  • ZHU Jia-Hong
    Key Laboratory of Rubber Biology, Ministry of Agriculture/State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
  • CHEN Xin
    Key Laboratory of Rubber Biology, Ministry of Agriculture/State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
  • CHANG Wen-Jun
    Key Laboratory of Rubber Biology, Ministry of Agriculture/State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences
  • TIAN Wei-Min
    Key Laboratory of Rubber Biology, Ministry of Agriculture/State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences
  • ZHANG Zhi-Li
    Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Hainan Academy of Agricultural Sciences

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  • Molecular Characterization of <I>HbCDPK1</I>, an Ethephon-Induced Calcium-Dependent Protein Kinase Gene of <I>Hevea brasiliensis</I>

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Calcium-dependent protein kinases (CDPKs), as major primary Ca2+ sensors, have been implicated in the regulation of stress and developmental signals in plants. In this study, a novel CDPK gene, designated HbCDPK1, was isolated from Hevea brasiliensis. The HbCDPK1 cDNA had 2,400 bp with an open reading frame of 1,671 bp encoding 556 amino acids, and the deduced HbCDPK1 protein contained four characteristic domains identified in CDPKs, showing a high level of sequence similarity to CDPKs from other plants. Expression analysis revealed more significant accumulation of the transcripts of HbCDPK1 in latex than in the leaves, bark, and roots in H. brasiliensis. In addition, transcription of HbCDPK1 was strongly induced by mechanical wounding, jasmonic acid (JA), and ethephon. Recombinant HbCDPK1 was expressed in E. coli, and its activity was assayed. The assay indicated that HbCDPK1 had the kinase and Ca2+-binding activity in vitro as a calcium-dependent protein. The potential roles of the HbCDPK1 are discussed as to latex production and rubber biosynthesis.

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