Characterization of Juvenile Hormone Epoxide Hydrolase and Related Genes in the Larval Development of the Silkworm Bombyx mori
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- SEINO Atsushi
- Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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- OGURA Takehiko
- Department of Applied Life Sciences, Kyoto University
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- TSUBOTA Takuya
- Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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- SHIMOMURA Masaru
- Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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- NAKAKURA Takayo
- Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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- TAN Anjiang
- Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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- MITA Kazuei
- Insect Genome Research Unit, National Institute of Agrobiological Science
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- SHINODA Tetsuro
- Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
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- NAKAGAWA Yoshiaki
- Department of Applied Life Sciences, Kyoto University
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- SHIOTSUKI Takahiro
- Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science
Bibliographic Information
- Other Title
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- Characterization of Juvenile Hormone Epoxide Hydrolase and Related Genes in the Larval Development of the Silkworm<i>Bombyx mori</i>
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Abstract
Juvenile hormone epoxide hydrolases (JHEHs) are a family of enzymes that hydrolyze juvenile hormones (JHs). They are important in terms of organ-specific regulation and irreversible degradation. In contrast to three JHEH genes (jheh) in Drosophila melanogaster and five jheh in Tribolium castaneum, only one jheh gene has been reported to date in lepidopteran insects. By searching a genome database of the silkworm, KAIKOBLAST, five JHEH-related genes (jheh-r), in addition to Bmjheh, were found. Developmental changes in mRNA expression were brought about revealing several unique patterns for each of jheh-r as to developmental stages and organ-specificity. Recombinant proteins of JHEH-r were expressed using a baculovirus system to evaluate their enzymatic activities. Three of the five JHEH-r recombinant proteins had JH hydrolytic activities. This is the first report on lepidopteran jheh-related genes and also provides the comprehensive analysis of multiple jheh-related genes in an insect species with respect to their functions in enzyme activities.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (7), 1421-1429, 2010
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282681456157312
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- NII Article ID
- 10027557293
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3cjgvFagtA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 10766605
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- PubMed
- 20622465
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed