TAS1<i>trans</i>-Acting siRNA Targets Are Differentially Regulated at Low Temperature, and TAS1<i>trans</i>-Acting siRNA Mediates Temperature-Controlled At1g51670 Expression

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  • KUME Kohei
    United Graduate School of Agricultural Sciences, Iwate University Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University
  • TSUTSUMI Ken-ichi
    United Graduate School of Agricultural Sciences, Iwate University Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University
  • SAITOH Yasushi
    United Graduate School of Agricultural Sciences, Iwate University Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University

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  • TAS1 trans-Acting siRNA Targets Are Differentially Regulated at Low Temperature, and TAS1 trans-Acting siRNA Mediates Temperature-Controlled At1g51670 Expression

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To endure considerable fluctuations in temperature, plants need precise regulation of temperature-controlled gene expression. In this study, the involvement of TAS1 trans-acting siRNA (tasiRNA) in temperature-controlled gene expression was examined in Arabidopsis. The accumulation of TAS1 tasiRNA was downregulated at 4 °C. Concomitant with the reduction of TAS1 tasiRNA-mediated cleavage, expression of At1g51670, a target of TAS1 tasiRNA, was upregulated at 4 °C in the wild type but not in a dicer-like enzyme (DCL) 4 mutant (dcl4-2), which is impaired in tasiRNA biogenesis. The expression of At4g29760 and of At5g18040, further TAS1 tasiRNA targets, was upregulated both in the wild type and in dcl4-2 at 4 °C. However, after shifting the temperature to 22 °C, low-temperature-induced expression of At4g29760 rapidly dropped in the wild type, but not in dcl4-2. Thus TAS1 tasiRNA acted as a sweeper for the clearance of excess amounts of At4g29760 transcripts. Our data suggest that differential regulation of TAS1 tasiRNA targets is involved in temperature-controlled gene expression.

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