Absolute Configuration of Hydroxycitric Acid Produced by Microorganisms

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  • HIDA Hiroyuki
    Department of Applied Biological Science, Faculty of Life Science and Biotechnology, Fukuyama University
  • YAMADA Takashi
    Department of Applied Biological Science, Faculty of Life Science and Biotechnology, Fukuyama University
  • YAMADA Yasuhiro
    Department of Applied Biological Science, Faculty of Life Science and Biotechnology, Fukuyama University

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Abstract

Optical resolution for (2S,3R) and (2R,3S)-hydroxycitric acid (HCA) enantiomers was developed using chiral column chromatography. HCA from Bacillus megaterium G45C and Streptomyces sp. U121, newly isolated in our previous study, was analyzed to determine the absolute configuration. These results indicate that both strains generate optically pure (2S,3R)-hibiscus type HCA enantiomer.

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