Enhancement of Thermal Stabilization of Formaldehyde Dehydrogenase from Pseudomonas putida by Directed Evolution

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  • Enhancement of Thermal Stabilization of Formaldehyde Dehydrogenase from<i>Pseudomonas putida</i>by Directed Evolution

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Abstract

We used directed evolution to enhance the thermostability of formaldehyde dehydrogenase from Pseudomonas putida. At 50 °C, the wild-type enzyme was inactivated within 30 min, but the variants obtained retained 80% activity for at least 300 min. At room temperature (30 °C), the variants obtained retained <80% activity for at least 500 h (21 d).

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