A Simple in Situ Cell-Based SUMOylation Assay with Potential Application to Drug Screening

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  • MURAMATSU Miyuki
    Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University
  • UWADA Junsuke
    Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University Global COE (Centers of Excellence) Program, Global Initiative Center for Pulsed Power Engineering, Kumamoto University
  • MATSUMOTO Naoyuki
    Faculty of Science, Kumamoto University
  • SAITOH Hisato
    Global COE (Centers of Excellence) Program, Global Initiative Center for Pulsed Power Engineering, Kumamoto University Faculty of Science, Kumamoto University Department of New Frontier Sciences, Graduate School of Science and Technology, Kumamoto University

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  • A Simple<i>in Situ</i>Cell-Based SUMOylation Assay with Potential Application to Drug Screening

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Abstract

We found that the small ubiquitin-related modifier (SUMO) conjugation reaction can be visualized simply by incubating green fluorescent protein (GFP)-SUMO-1 with permeabilized cells in the presence of ATP for 15 min. Neither special equipment for protein purification nor highly developed skills for recombinant technologies is required, making the assay potentially applicable to large-scale drug-screening strategies for the identification of drugs that can inhibit or enhance SUMOylation.

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