Lack of the Consensus Sequence Necessary for Tryptophan Prenylation in the ComX Pheromone Precursor
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- TSUJI Fumitada
- Graduate School of Bioagricultural Sciences, Nagoya University
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- ISHIHARA Ayako
- Graduate School of Bioagricultural Sciences, Nagoya University
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- NAKAGAWA Aya
- Graduate School of Bioagricultural Sciences, Nagoya University
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- OKADA Masahiro
- Graduate School of Bioscience and Biotechnology, Chubu University
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- KITAMURA Shigeyuki
- Graduate School of Bioagricultural Sciences, Nagoya University
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- KANAMARU Kyoko
- Graduate School of Bioagricultural Sciences, Nagoya University
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- MASUDA Yuichi
- Graduate School of Agriculture, Kyoto University
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- MURAKAMI Kazuma
- Graduate School of Agriculture, Kyoto University
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- IRIE Kazuhiro
- Graduate School of Agriculture, Kyoto University
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- SAKAGAMI Youji
- Graduate School of Bioagricultural Sciences, Nagoya University
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抄録
ComX, an oligopeptide pheromone that stimulates the natural genetic competence controlled by quorum sensing in Bacillus subtilis and related bacilli, contains a prenyl-modified tryptophan residue. Since ComX is the only protein known to contain prenylated tryptophan, the universality of this unique posttranslational modification has yet to be determined. Recently, we developed a cell-free assay system in which the tryptophan residue in the ComXRO-E-2 pheromone precursor derived from B. subtilis strain RO-E-2 can be geranylated by the ComQRO-E-2 enzyme. We report here our attempt to identify the consensus sequence surrounding the geranylated tryptophan residue by using the cell-free system with various ComXRO-E-2 pheromone precursor analogs. We found that [47–58]ComXRO-E-2, corresponding to the C-terminal 12-residue peptide of the pheromone precursor, contained a short sequence essential for geranylation. We also found that the length of the sequence between the tryptophan residue and the C-terminus was important for geranylation, and that some [47–58]ComXRO-E-2 pheromone precursor amino acids were involved in the geranylation reaction. However, we could not identify a consensus sequence surrounding the geranylated tryptophan. Our evidence suggests that, like Rab which lacks a consensus sequence yet is geranylgeranyl-modified on a cysteine residue, the ComX pheromone and its precursor also lack a consensus sequence.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (8), 1492-1496, 2012
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456253568
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- NII論文ID
- 10031065914
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC38fmslOmuw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 023915671
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- PubMed
- 22878193
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可