High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

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  • TAKAHASHI Saori
    Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center
  • HATA Keishi
    Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center
  • KIKUCHI Ken-Ichi
    Department of Materials-Process Engineering and Applied Chemistry for the Environment, Akita University
  • GOTOH Takeshi
    Department of Materials-Process Engineering and Applied Chemistry for the Environment, Akita University

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Abstract

Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.

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