Archaeal Aldehyde Dehydrogenase ST0064 from <i>Sulfolobus tokodaii</i>, a Paralog of Non-Phosphorylating Glyceraldehyde-3-phosphate Dehydrogenase, Is a Succinate Semialdehyde Dehydrogenase
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- ITO Fumiaki
- Department of Biotechnology, The University of Tokyo
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- CHISHIKI Hidehiro
- Department of Biotechnology, The University of Tokyo
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- FUSHINOBU Shinya
- Department of Biotechnology, The University of Tokyo
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- WAKAGI Takayoshi
- Department of Biotechnology, The University of Tokyo
Bibliographic Information
- Other Title
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- Archaeal Aldehyde Dehydrogenase ST0064 from Sulfolobus tokodaii, a Paralog of Non-Phosphorylating Glyceraldehyde-3-phosphate Dehydrogenase, Is a Succinate Semialdehyde Dehydrogenase
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Abstract
Aldehyde dehydrogenase ST0064, the closest paralog of previously characterized allosteric non-phosphorylating glyceraldehyde-3-phosphate (GAP) dehydrogenase (GAPN, ST2477) from a thermoacidophilic archaeon, Sulfolobus tokodaii, was expressed heterologously and characterized in detail. ST0064 showed remarkable activity toward succinate semialdehyde (SSA) (Km of 0.0029 mM and kcat of 30.0 s−1) with no allosteric regulation. Activity toward GAP was lower (Km of 4.6 mM and kcat of 4.77 s−1), and previously predicted succinyl-CoA reductase activity was not detected, suggesting that the enzyme functions practically as succinate semialdehyde dehydrogenase (SSADH). Phylogenetic analysis indicated that archaeal SSADHs and GAPNs are closely related within the aldehyde dehydrogenase superfamily, suggesting that they are of the same origin.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (6), 1344-1348, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681456478592
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- NII Article ID
- 10031184711
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- NII Book ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sjhtFWntw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 024647337
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- PubMed
- 23748791
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed