Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic <i>N</i>-Glycan
-
- MAEDA Megumi
- Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University
-
- TAKEDA Naoto
- Faculty of Agriculture, Division of Agricultural Science, Okayama University
-
- MANO Aya
- Faculty of Agriculture, Division of Agricultural Science, Okayama University
-
- YAMANISHI Maomi
- Faculty of Agriculture, Division of Agricultural Science, Okayama University
-
- KIMURA Mariko
- Faculty of Food Culture, Department of Nutrition, Kurashiki Sakuyo University Faculty of Home Economics, Kobe Women's University
-
- KIMURA Yoshinobu
- Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University
Bibliographic Information
- Other Title
-
- Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic N-Glycan
Search this article
Description
In our previous report (M. Okano, et al., Clin. Exp. Allergy, 34, 770–778 (2004)), we found that free plant complex type N-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type N-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type N-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asn-glycopeptide bearing plant complex type N-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103 mg of Asn-glycopeptide bearing the antigenic N-glycans was obtained from 1.9 kg of shelled Ginkgo biloba seeds.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 77 (6), 1269-1274, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282681456499072
-
- NII Article ID
- 10031184693
-
- NII Book ID
- AA10824164
-
- COI
- 1:STN:280:DC%2BC3sjhtFWntg%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 024647125
-
- PubMed
- 23748790
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed