Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic <i>N</i>-Glycan

  • MAEDA Megumi
    Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University
  • TAKEDA Naoto
    Faculty of Agriculture, Division of Agricultural Science, Okayama University
  • MANO Aya
    Faculty of Agriculture, Division of Agricultural Science, Okayama University
  • YAMANISHI Maomi
    Faculty of Agriculture, Division of Agricultural Science, Okayama University
  • KIMURA Mariko
    Faculty of Food Culture, Department of Nutrition, Kurashiki Sakuyo University Faculty of Home Economics, Kobe Women's University
  • KIMURA Yoshinobu
    Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University

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  • Large-Scale Preparation of Asn-Glycopeptide Carrying Structurally Homologous Antigenic N-Glycan

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In our previous report (M. Okano, et al., Clin. Exp. Allergy, 34, 770–778 (2004)), we found that free plant complex type N-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type N-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type N-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asn-glycopeptide bearing plant complex type N-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103 mg of Asn-glycopeptide bearing the antigenic N-glycans was obtained from 1.9 kg of shelled Ginkgo biloba seeds.

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