Quantification of Major Royal Jelly Protein 1 in Fresh Royal Jelly by Indirect Enzyme-Linked Immunosorbent Assay

  • YAMAGUCHI Kikuji
    College of Plant Protection, Yunnan Agricultural University Head office, Japan Royal Jelly Co., Ltd.
  • HE Shaoyu
    College of Plant Protection, Yunnan Agricultural University Eastern Bee Institute, Yunnan Agricultural University
  • LI Zhengyue
    College of Plant Protection, Yunnan Agricultural University
  • MURATA Kiyoshi
    R&D Center, Japan Royal Jelly Co., Ltd.
  • HITOMI Nobuyuki
    R&D Center, Japan Royal Jelly Co., Ltd.
  • MOZUMI Manaho
    Department of Food Science, Ishikawa Prefectural University
  • ARIGA Risa
    Department of Food Science, Ishikawa Prefectural University
  • ENOMOTO Toshiki
    Department of Food Science, Ishikawa Prefectural University

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抄録

Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12–4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.

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