Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research

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  • NAKAGAWA Tsuyoshi
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
  • NAKAMURA Shinya
    Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
  • TANAKA Katsunori
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
  • KAWAMUKAI Makoto
    Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
  • SUZUKI Takamasa
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
  • NAKAMURA Kenzo
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
  • KIMURA Tetsuya
    Department of Sustainable Resource Science, Faculty of Bioresources, Mie University
  • ISHIGURO Sumie
    Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University

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Other Title
  • Development of R4 Gateway binary bectors (R4pGWB) enabling high-throughput promoter swapping for plant research
  • Development of R4 gateway binary vectors (R4pGWB) enabling high‐throughput promoter swapping for plant research
  • Development of R4 gateway binary vectors (R4pGWB) enabling high-throughput promoter swapping for plant tesearch
  • Development of R4 gateway binary vectors (R4pGWB) enabling high-throughput promoter swapping for plant research, Bioscience

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We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA. The structure of R4pGWBs is almost the same as the promoterless type of improved pGWBs (ImpGWBs), except that the attR1 site is replaced with attR4, which enables tripartite recombination of these vectors with promoter- and cDNA-entry clones. While ImpGWBs are suitable for promoter analysis and constitutive expression of cDNAs in higher plants, R4pGWBs have a great advantage in expressing a cDNA under the regulation of desired promoters.

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