Novel Promoter Sequence Required for Inductive Expression of the Aspergillus nidulans Endoglucanase Gene eglA

  • ENDO Yoshikazu
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • YOKOYAMA Misako
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • MORIMOTO Munenori
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • SHIRAI Kengo
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • CHIKAMATSU Go
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • KATO Naoki
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • TSUKAGOSHI Norihiro
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • KATO Masashi
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • KOBAYASHI Tetsuo
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

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  • Novel Promoter Sequence Required for Inductive Expression of the<i>Aspergillus nidulans</i>Endoglucanase Gene<i>eglA</i>

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Abstract

Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus.<BR>Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning −153 to −138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.

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