Denitrification by the Fungus<i>Fusarium oxysporum</i>Involves NADH-Nitrate Reductase
-
- FUJII Tatsuya
- Graduate School of Life and Environmental Sciences, University of Tsukuba
-
- TAKAYA Naoki
- Graduate School of Life and Environmental Sciences, University of Tsukuba
書誌事項
- タイトル別名
-
- Denitrification by the Fungus Fusarium oxysporum Involves NADH-Nitrate Reductase
この論文をさがす
説明
Fusarium oxysporum JCM11502 expresses a denitrifying (nitrate (NO3−)-respiring) mechanism and can thrive under oxygen (O2) limitation. The fungus reduces NO3− to nitrite at the initial step of denitrification. In this study, we cloned the gene coding NADH-NO3− reductase (NADH-Nar) (niaD) from F. oxysporum JCM11502. The niaD gene complemented the defective NO3− assimilation by mutant strain M10, indicating that the fungus reduced NO3− through NADH-Nar activity and assimilated it like other fungi. We found that the transcription of niaD and the production of NADH-Nar activity were enhanced under O2-limited denitrifying conditions relative to aerobic conditions. Strain M10 produced less NADH-Nar activity and less denitrified product than the wild-type strain. Introducing niaD into the mutant also restored these defects, indicating that niaD is involved in denitrification. These results indicate that the fungus denitrified NO3− through NADH-Nar activity in addition to the ubiquinol-Nar mechanism.
収録刊行物
-
- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
-
Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (2), 412-420, 2008
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681456828928
-
- NII論文ID
- 10027524224
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 9410107
-
- PubMed
- 18256499
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
- OpenAIRE
-
- 抄録ライセンスフラグ
- 使用不可
