Overproduction of Hyperthermostable β-1,4-Endoglucanase from the Archaeon <i>Pyrococcus horikoshii</i> by Tobacco Chloroplast Engineering

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  • NAKAHIRA Yoichi
    Venture Business Laboratory, Ehime University Graduate School of Life and Environmental Sciences Kyoto Prefectural University Phytoculture Control Co., Ltd.
  • ISHIKAWA Kazuhiko
    Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST)
  • TANAKA Kunisuke
    Graduate School of Life and Environmental Sciences Kyoto Prefectural University Phytoculture Control Co., Ltd.
  • TOZAWA Yuzuru
    Venture Business Laboratory, Ehime University Proteo-Science Center, Ehime University
  • SHIINA Takashi
    Graduate School of Life and Environmental Sciences Kyoto Prefectural University

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  • Overproduction of Hyperthermostable β-1,4-Endoglucanase from the Archaeon Pyrococcus horikoshii by Tobacco Chloroplast Engineering

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Abstract

One of the most cost-effective methods of producing industrial enzymes is by the use of transgenic plants. We demonstrated successful high-level expression of a hyperthermostable archaeal β-1,4-endoglucanase in mature tobacco leaves by transformation of chloroplasts by homologous recombination. The active recombinant enzyme was readily recovered not only from fresh but also from dried leaves.

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