Novel Exopolysaccharides Produced by Lactococcus lactis subsp. lactis, and the Diversity of epsE Genes in the Exopolysaccharide Biosynthesis Gene Clusters
-
- SUZUKI Chise
- NARO Institute of Livestock and Grassland Science
-
- KOBAYASHI Miho
- NARO Institute of Livestock and Grassland Science
-
- KIMOTO-NIRA Hiromi
- NARO Institute of Livestock and Grassland Science
書誌事項
- タイトル別名
-
- Novel Exopolysaccharides Produced by <i>Lactococcus lactis</i> subsp. <i>lactis</i>, and the Diversity of <i>epsE</i> Genes in the Exopolysaccharide Biosynthesis Gene Clusters
この論文をさがす
抄録
To characterize novel variations of exopolysaccharides (EPSs) produced by dairy strains of Lactococcus lactis subsp. lactis and subsp. cremoris, the EPSs of five dairy strains of L. lactis were purified. Sugar composition analysis showed two novel EPSs produced by strains of L. lactis subsp. lactis. One strain produced EPS lacking galactose, and the other produced EPS containing fucose. Among the eps gene clusters of these strains, the highly conserved epsD and its neighboring epsE were sequenced. Sequence and PCR analysis revealed that epsE genes were strain-specific. By Southern blot analysis using epsD, the eps gene cluster in each strain was found to locate to the chromosome or a very large plasmid. This is the first report on the identification of two novel EPSs in L. lactis subsp. lactis. The strains can be detected among other strains by using epsE genes specific to them.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 77 (10), 2013-2018, 2013
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681456871168
-
- NII論文ID
- 130003381881
-
- NII書誌ID
- AA10824164
-
- COI
- 1:STN:280:DC%2BC2c%2FjtFOquw%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 024971916
-
- PubMed
- 24096663
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可