Evidence of Antagonistic Regulation of Restart from G₁ Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast
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- MIZUNUMA Masaki
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- OGAWA Takafumi
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- KOYAMA Tetsuya
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- SHITAMUKAI Atsunori
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- TSUBAKIYAMA Ryohei
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- KOMARUYAMA Tadamasa
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- YAMAGUCHI Toshinaga
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- KUME Kazunori
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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- HIRATA Dai
- Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
書誌事項
- タイトル別名
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- Evidence of Antagonistic Regulation of Restart from G<sub>1</sub> Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast
この論文をさがす
抄録
Hog1 of Saccharomyces cerevisiae is activated by hyperosmotic stress, and this leads to cell-cycle delay in G1, but the mechanism by which cells restart from G1 delay remains elusive. We found that Whi3, a negative regulator of G1 cyclin, counteracted Hog1 in the restart from G1 delay caused by osmotic stress. We have found that phosphorylation of Ser-568 in Whi3 by RAS/cAMP-dependent protein kinase (PKA) plays an inhibitory role in Whi3 function. In this study we found that the phosphomimetic Whi3 S568D mutant, like the Δwhi3 strain, slightly suppressed G1 delay of Δhog1 cells under osmotic stress conditions, whereas the non-phosphorylatable S568A mutation of Whi3 caused prolonged G1 arrest of Δhog1 cells. These results indicate that Hog1 activity is required for restart from G1 arrest under osmotic stress conditions, whereas Whi3 acts as a negative regulator for this restart mechanism.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (10), 2002-2007, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456874240
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- NII論文ID
- 130003381877
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC2c%2FjtFOqtw%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024971880
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- PubMed
- 24096659
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可