Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of Miscanthus sinensis to Monosaccharides

YOSHIDA Makoto, LIU Yuan, UCHIDA Satoshi, KAWARADA Kensuke, UKAGAMI Yusuke, ICHINOSE Hitomi, KANEKO Satoshi, FUKUDA Kiyoharu

doi:10.1271/bbb.70689

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Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of<i>Miscanthus sinensis</i>to Monosaccharides
Effects of cellulose crystallinity, hemisellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides
Effect of cellulose crystallinity, hemicelluloses, and lignin on enzymatic hydrolysis of Miscanthus sinesis to monosaccharides

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The effects of cellulose crystallinity, hemicellulose, and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides were investigated. A air-dried biomass was ground by ball-milling, and the powder was separated into four fractions by passage through a series of sieves with mesh sizes 250–355 μm, 150–250 μm, 63–150 μm, and <63 μm. Each fraction was hydrolyzed with commercially available cellulase and β-glucosidase. The yield of monosaccharides increased as the crystallinity of the substrate decreased. The addition of xylanase increased the yield of both pentoses and glucose. Delignification by the sodium chlorite method improved the initial rate of hydrolysis by cellulolytic enzymes significantly, resulting in a higher yield of monosaccharides as compared with that for untreated samples. When delignified M. sinensis was hydrolyzed with cellulase, β-glucosidase, and xylanase, hemicellulose was hydrolyzed completely into monosaccharides, and the conversion rate of glucan to glucose was 90.6%.

Journal

Bioscience, Biotechnology, and Biochemistry

Bioscience, Biotechnology, and Biochemistry 72 (3), 805-810, 2008

Japan Society for Bioscience, Biotechnology, and Agrochemistry

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