Effects of Ala Substitution for Conserved Cys Residues in Mouse Ileal and Hepatic Na<sup>+</sup>-Dependent Bile Acid Transporters
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- SAEKI Tohru
- Laboratory of Molecular Nutrition, Kyoto Prefectural University
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- MUNETAKA Yuki
- Laboratory of Molecular Nutrition, Kyoto Prefectural University
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- UEDA Kazumitsu
- Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
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- IWAMI Kimikazu
- Laboratory of Molecular Nutrition, Kyoto Prefectural University
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- KANAMOTO Ryuhei
- Laboratory of Molecular Nutrition, Kyoto Prefectural University
書誌事項
- タイトル別名
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- Effects of Ala Substitution for Conserved Cys Residues in Mouse Ileal and Hepatic Na+-Dependent Bile Acid Transporters
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抄録
Although ileal and hepatic Na+-dependent bile acid transporters (SLC10A2 and SLC10A1 respectively) share structural similarities, the mutation of conserved amino acids often has distinct effects on them. We have identified two Cys residues in mouse Slc10a2 (Cys51 and Cys106) the replacement of which by Ala remarkably reduces taurocholic acid (TCA) transport. Although Cys51 is conserved in Slc10a1 as Cys44, Ala substitution gave no apparent difference in TCA uptake. Here, we further analyzed the kinetics of TCA uptake and cell surface localization of these mutants. The C51A and C106A mutants of Slc10a2 showed significantly reduced TCA uptake, while no apparent difference in TCA uptake was observed for the Slc10a1-C44A mutant. The Km values for TCA uptake by these mutants were comparable, suggesting that these residues are not involved in the interaction with TCA.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (8), 1865-1872, 2007
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456993536
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- NII論文ID
- 10027517327
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8887324
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可