Site-Specific and Asymmetric Hydrolysis of Prochiral 2-Phenyl-1,3-propanediol Diacetate by a Bacterial Esterase from an Isolated Strain
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- MITSUI Ryoji
- Department of Biochemistry, Faculty of Science, Okayama University of Science
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- SHINYA Seiho
- Department of Biochemistry, Faculty of Science, Okayama University of Science
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- ICHIYAMA Yuichi
- Department of Biochemistry, Faculty of Science, Okayama University of Science
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- KUDO Kenta
- Department of Biochemistry, Faculty of Science, Okayama University of Science
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- TSUNO Takuo
- Tsuno Food Industrial Co., Ltd.
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- TANAKA Mitsuo
- Department of Biochemistry, Faculty of Science, Okayama University of Science
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Bacillus cereus 809A and Burkholderia sp. 711C were isolated from soil. These strains demonstrate hydrolysis activity towards prochiral 2-phenyl-1,3-propanediol diacetate and accumulated the corresponding chiral monoacetates into the reaction mixture. When 2-phenyl 1,3-propanediol diacetate was used as a substrate, the produced monoacetates with Burkholderia sp. 711C were obtained in a racemic form but that produced by Bacillus cereus 809A showed an excess of the (S)-form. The resting cell reaction revealed that for Bacillus cereus 809A, there was an enrichment of one of the enantiomers of the monoacetate such that the enantiomeric excess (e.e.) of the (S)-form was over 95%. The purified enzyme from Bacillus cereus 809A hydrolyzed diacetate to monoacetate, and the e.e. value of the (S)-form increased by prolonged reaction in a way similar to the resting cell reaction. From N-terminal amino acids, this esterase is conserved in some strains of Bacillus for which the genomic sequences have been reported.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (8), 1858-1864, 2007
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456995712
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- NII論文ID
- 10027517309
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8887240
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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