Proteomic Analysis of the Anti-Cancer Effect of 20<i>S</i>-Ginsenoside Rg<sub>3</sub>in Human Colon Cancer Cell Lines

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  • LEE Seo Young
    College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • KIM Geun Tae
    College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • ROH Si Hun
    College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • SONG Jin-Su
    Department of Chemistry, Seoul National University
  • KIM Hie-Joon
    Department of Chemistry, Seoul National University
  • HONG Soon-Sun
    College of Medicine (BK21), Inha University
  • KWON Sung Won
    College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University
  • PARK Jeong Hill
    College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University

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  • Proteomic Analysis of the Anti-Cancer Effect of 20S-Ginsenoside Rg3 in Human Colon Cancer Cell Lines

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Ginseng is a well known herbal medicine in Asia, and ginsenoside Rg3 has anti-cancer and various pharmacological effects. In particular, 20S-ginsenoside Rg3 may increase the anti-proliferative effects of chemotherapy. The authors investigated the mechanism of the anti-proliferative effect of 20S-Rg3 at the protein level in HT29 colon cancer cells. MTT, caspase-3 assays, and flow cytometry analysis were performed to determine cytotoxicity and apoptosis, and proteomic analysis was performed by two-dimensional gel electrophoresis and MALDI-TOF/TOF MS, and a database was used to identify protein changes in 20S-Rg3 treated HT29 cells. The proteins identified included down-regulated Rho GDP dissociation inhibitor, up-regulated tropomyosin1, and annexin5 and glutathione s-transferase p1, which are apoptosis associated proteins. The anti-proliferative mechanism of 20S-Rg3 was found to be involved in mitotic inhibition, DNA replication, and repair and growth factor signaling. The findings of this study suggest that the cytotoxicity of 20S-Rg3 in colon cancer is dependent on several mechanisms, including apoptosis.

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