Exploring Amino Acids Responsible for the Temperature Profile of Glycoside Hydrolase Family 45 Endoglucanase EGL3 from<i>Humicola grisea</i>
-
- MURASHIMA Koichiro
- Food and Health R & D Laboratories, Meiji Seika Kaisha, Ltd.
-
- SHIMONAKA Atsushi
- Food and Health R & D Laboratories, Meiji Seika Kaisha, Ltd.
-
- NISHIMURA Tomoko
- Food and Health R & D Laboratories, Meiji Seika Kaisha, Ltd.
-
- BABA Yuko
- Food and Health R & D Laboratories, Meiji Seika Kaisha, Ltd.
-
- KOGA Jinichiro
- Food and Health R & D Laboratories, Meiji Seika Kaisha, Ltd.
-
- KUBOTA Hidetoshi
- Food and Health R & D Laboratories, Meiji Seika Kaisha, Ltd.
-
- KONO Toshiaki
- Food and Health R & D Laboratories, Meiji Seika Kaisha, Ltd.
書誌事項
- タイトル別名
-
- Exploring Amino Acids Responsible for the Temperature Profile of Glycoside Hydrolase Family 45 Endoglucanase EGL3 from Humicola grisea
この論文をさがす
説明
EGL3 and RCE1 are glycoside hydrolase family 45 endoglucanases isolated from Humicola grisea and Rhizopus oryzae respectively. The amino acid sequences of the two endoglucanases are homologous; on the other hand, the optimum temperature of EGL3 is higher than that of RCE1. In this study, four chimeric endoglucanases, named ER1, ER2, ER3 and ER4, in which one of four sequential amino acid regions of the EGL3 catalytic domain (CAD) was replaced by the corresponding RCE1 amino acids, were constructed to explore the region responsible for the EGL3 temperature profile. Then their temperature profiles were compared with that of the recombinant EGL3. Replacement of the N-terminal region of EGL3 with that of RCE1 caused the EGL3 temperature profile to shift to a lower temperature. These results suggest that the N-terminal amino acids of the EGL3 are responsible for the EGL3 temperature profile.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 70 (9), 2205-2212, 2006
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681457303680
-
- NII論文ID
- 130000029740
-
- NII書誌ID
- AA10824164
-
- COI
- 1:STN:280:DC%2BD28rntlKisg%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 8073541
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可