Characterization of [1-13C]-Specifically Labeled Amino Acids Using ESIMS/MS in the Selected Reaction Monitoring Mode
-
- OHASHI Yoko
- Department of Applied Physics and Chemistry, The University of Electro-Communications
-
- HATASE Hiroshi
- Department of Applied Physics and Chemistry, The University of Electro-Communications
-
- NAKAMURA Mitsuhiro
- Department of Applied Physics and Chemistry, The University of Electro-Communications
-
- HIRANO Takashi
- Department of Applied Physics and Chemistry, The University of Electro-Communications
-
- MAKI Shojiro
- Department of Applied Physics and Chemistry, The University of Electro-Communications
-
- NIWA Haruki
- Department of Applied Physics and Chemistry, The University of Electro-Communications
Search this article
Description
ESIMS/MS was employed for the analyses of 1-13CO-labeled amino acids. Conventionally, in order to estimate the 13C-content, the intensity of molecule-related ion of the sample has been compared against the natural compound. However, this method is incompetent for specifying the position of the 13C-labeling, hence is unable to distinguish the specifically 1-13C-labeled compound from otherwise-(generally or non-specifically) labeled amino acids. With the intention of distinguishing 1-13CO-labeled amino acids from generally labeled and non-labeled molecules we utilized ESIMS/MS in the selected reaction monitoring mode, choosing the protonated molecules as the precursor ion and specifying the product ion generated by a 47 u- (instead of 46 u-) elimination from the precursor ion for the labeled samples. By this new method, characterization of solely 1-13CO-labeled amino acids has been successfully conducted, enabling their distinction from otherwise-labeled compounds.
Journal
-
- Journal of the Mass Spectrometry Society of Japan
-
Journal of the Mass Spectrometry Society of Japan 53 (6), 309-314, 2005
The Mass Spectrometry Society of Japan
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282681469082496
-
- NII Article ID
- 10017073391
-
- NII Book ID
- AN0010555X
-
- COI
- 1:CAS:528:DC%2BD28Xkt1Sitw%3D%3D
-
- ISSN
- 18804225
- 18843271
- 13408097
-
- NDL BIB ID
- 7762030
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL Search
- Crossref
- CiNii Articles
- OpenAIRE
-
- Abstract License Flag
- Disallowed