A study on the utility of ultrasonic treatment for preparing fossil diatom slides

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  • Hirose Kotaro
    Graduate School of Symbiotic Systems Science and Technology, Fukushima University
  • Yoshioka Kaoru
    Department of Materials Creation and Circulation Technology, Interdisciplinary Graduate School of Science and Engineering, Shimane University
  • Irizuki Toshiaki
    Department of Geoscience, Interdisciplinary Faculty of Science and Engineering, Shimane University
  • Iwai Masao
    Faculty of Science, Kochi University
  • Gotoh Toshikazu
    Division of Basic Medical Science, Faculty of Medicine, Kinki University

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Other Title
  • 超音波印加による珪藻分析のための簡便な堆積物処理法
  • チョウオンパ インカ ニ ヨル ケイソウ ブンセキ ノ タメ ノ カンベン ナ タイセキブツ ショリホウ

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Abstract

We verified the significance of a simple and easy treatment using ultrasonic cleaner, which was designed to minimize possible modification of original diatom assemblages preserved in sediments. Our ultrasonic treatment demonstrated that diatom valves incurred little damage ; in addition, this treatment was effective to disaggregate the consolidate structure mainly of fecal pellets. Comparing the diatom assemblages in the samples treated by hydrogen peroxide (an established method for diatom slide preparation) with those processed by ultrasonication, we concluded that the ultrasonic treatment provides a better result in preserving micro and fragile valves that otherwise easily flow out while captured in supernatant during the dacantation process of the hydrogen peroxide method. Furthermore, the investigation of the difference between diatom assemblages in aggregate structures (mainly of fecal pellets) and those in sediment matrix suggests the possibility that diatom analysis of community structures in fecal pellets tends to reconstruct the population dynamics of consumers whose fossils are known to be rarely preserved in underwater sediment.

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