書誌事項
- タイトル別名
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- Sample Preparation Methods for Large-Scale Analysis of Membrane Proteins Using Shotgun Proteomics
- ショットガンプロテオミクス ニ ヨル マク タンパクシツ ノ モウラテキ カイセキ ノ タメ ノ シリョウ チョウセイホウ
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説明
Shotgun proteomics based on liquid chromatography/mass spectrometry has been widely used to provide a large-scale view of the proteome, including protein expression, localization, modification, and protein-protein interaction. However, it is still challenging tasks to carry out membrane protein-targeted proteome analysis due to the hydrophobic nature of proteins with transmembrane domains. Here, we review current sample preparation techniques such as enrichment, solubilization, and digestion for membrane proteomics and focus on phase-transfer surfactant-based approaches recently developed by our group. We also discuss future developments for membrane proteome focused shotgun proteomics.
収録刊行物
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- 質量分析
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質量分析 57 (3), 145-151, 2009
一般社団法人 日本質量分析学会
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詳細情報 詳細情報について
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- CRID
- 1390282681472460416
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- NII論文ID
- 10024837589
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- NII書誌ID
- AN0010555X
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- ISSN
- 18804225
- 18843271
- 13408097
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- NDL書誌ID
- 10263883
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- 本文言語コード
- ja
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可
