Improvement of Detectable Sensitivity for Enzyme Reaction by Scanning Electrochemical Microscopy with Distance Control System for Immunosensing
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- HIRANO Yu
- National Institute of Advanced Industrial, Science and Technology (AIST)
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- YASUKAWA Tomoyuki
- Graduate School of Material Science, University of Hyogo
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- MASE Yoshiaki
- Graduate School of Environmental Studies, Tohoku University
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- OYAMATSU Daisuke
- Graduate School of Environmental Studies, Tohoku University
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- SHIKU Hitoshi
- Graduate School of Environmental Studies, Tohoku University
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- MIZUTANI Fumio
- Graduate School of Material Science, University of Hyogo
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- MATSUE Tomokazu
- Graduate School of Environmental Studies, Tohoku University
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Detectable sensitivity for immobilized enzymes was improved using a constant-distance mode of scanning electrochemical microscopy (SECM). Redox species generated by an enzyme reaction captured via immunorecognition were detected with a microelectrode used as a probe. The probe maintained considerably close to the target surface (50 nm) allowed the enhancement of the current response because of cycling of redox species between the probe and the captured enzymes. The sensitivity of di(n-butyl)phthalate detection with a competitive assay by using constant-distance mode SECM was at least one order of magnitude higher than that of the probe scanned in a constant z-position.
収録刊行物
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- Electrochemistry
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Electrochemistry 80 (1), 30-32, 2012
公益社団法人 電気化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681472700544
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- NII論文ID
- 10029981363
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- NII書誌ID
- AN00151637
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- ISSN
- 21862451
- 13443542
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- NDL書誌ID
- 023389585
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用可
