Electrochemical Detection of Vascular Endothelial Growth Factor with Aptamer Sandwich

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  • NONAKA Yoshihiko
    Graduate school of engineering, Tokyo University of Agriculture and Technology
  • ABE Koichi
    Graduate school of engineering, Tokyo University of Agriculture and Technology
  • IKEBUKURO Kazunori
    Graduate school of engineering, Tokyo University of Agriculture and Technology

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We developed an electrochemical vascular endothelial growth factor (VEGF) detection system using two VEGF-binding aptamers. Sensitive detection systems of VEGF are highly required for cancer diagnosis. In this study, VEGF-A was detected using a sandwiching method with a pyrroquinoline quinone glucose dehydrogenase (PQQ-GDH)-labeled VEGF-binding aptamer and another aptamer immobilized onto a gold wire electrode. We evaluated various combinations of VEGF-binding aptamers to be employed in the process. In addition, we examined dose dependency for the electric current generated by PQQ-GDH, measured in the constructed VEGF detection system. Using this newly constructed system, we successfully detected VEGF165 at 15 nM (M = mol dm−3) concentration.

収録刊行物

  • Electrochemistry

    Electrochemistry 80 (5), 363-366, 2012

    公益社団法人　電気化学会

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