Probing of Structure and Function of Proteins by Mass Spectrometry -Analysis of Structure and Function of a Protein by the pH Titration Using ESI-MS-

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  • タンパク質の構造と機能を質量分析で解明する　　―ＥＳＩ－ＭＳによるｐＨ滴定でタンパク質の構造と機能を解析する―
  • タンパクシツ ノ コウゾウ ト キノウ オ シツリョウ ブンセキ デ カイメイ スル ESI MS ニ ヨル pH テキテイ デ タンパクシツ ノ コウゾウ ト キノウ オ カイセキ スル
  • —Analysis of Structure and Function of a Protein by the pH Titration Using ESI-MS—
  • —ESI-MSによるpH滴定でタンパク質の構造と機能を解析する—

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An analysis of the pH-dependent dissociation and denaturation of a heterodimeric protein is described. Electrospray ionization mass spectrometry (ESI-MS) was utilized to analyze the dissociation and denaturation of the heterodimeric yeast killer toxin SMKT. The two distinct subunits of SMKT noncovalently associate with one another under acidic conditions, but dissociate and are then denatured under neutral and basic conditions. In order to understand the mechanism that controls the unique pH-dependent denaturation mechanism of this protein, a pH titration was performed using ESI-MS. In this report, the MS conditions for the pH-titration and data-analysis procedures are described.

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